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Our current analyses
are dissolved free amino acids (DFAA); protein (as total hydrolyzable
amino acids by HPLC) in seawater, particulate material, sediment, and
biological material; C:37 alkenones; biogenic opal in particulate
material and sediment; phytoplankton nutrients (nitrate, nitrite,
silicate, phosphate, ammonia); photosynthetic pigments and derivatives;
proteins in biological material using the Coomassie Blue assay (manual
by Bio-Rad). We are currently purifying chlorins for N isotope
analysis from particles and sediments, and implementing the technique
for analyzing low-molecular weight organic acids (Alberts and Martens
1997).
Facilities in my lab include 2 HPLC's (one with a photodiode array
UV-VIS detector, dedicated to the extraction and purification of
chlorins for isotopic analysis), another HPLC equipped with a
fluorescence detector and robotic autosampler mainly used for amino
acid and amine determination, a Gas Chromatograph with FID detector, a
Gas Chromatograph with both Mass Selective Detector and FID, and a
UV-VIS spectrophotometer with a robotic autosampler for analyses of
biogenic and lithogenic opal, dissolved inorganic nutrients, protein
(using the Coomassie Blue assay), and dissolved organic nitrogen (DON).
Other instruments are a refrigerated centrifuge, and a Milli-Q water
system, and extensive minor analytical and preparatory equipment.
Field research is supported by our expandable (2-6m) sediment gravity
corer, drifting sediment traps and oceanographic bottles. |
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